TABLA II. Condiciones de tipificación mediante PCR-RFLP
Gen |
Polimorfismo |
Cebadores |
Condicionesª PCRºC |
Enzimas Restricción |
Alelo |
Tamaño Fragmentos |
|
|
|
|
|
|
|
IL13 |
C-1112T |
5’ GGAATCCAGCATGCCTTGTGAGG 3’ 5’ GTCGCCTTTTCCTGCTCTTCCCGC 3’ |
54 |
BstUI (0.2U) |
C |
224 |
|
|
|
|
|
T |
247 |
IL4RA |
I50V |
5’ GGCAGGTGTGAGGAGCATCC 3’ 5’ GCCTCCGTTGTTCTCAGGTA 3’ |
60 |
RsaI (1U) |
A |
254 |
|
|
|
|
|
G |
273 |
B2AR |
R16G |
5’ CCTTCTTGCTGGCACCCCAT 3’ 5’ CCGTCTGCAGACGCTCGAAC 3’ |
60 |
NcoI (2U) |
G |
174 |
|
|
|
|
|
A |
157 |
|
Q27E |
5’ GGCCCATGACCAGATCAGCA 3’ 5’ GAATGAGGCTTCCAGGCGTC 3’ |
60 |
Fnu4HI (0.4U) |
G |
174 |
|
|
|
|
|
C |
229 |